Observation of Fixative Process for the Isolated Hair Cells from the Guinea Pig Cochlea
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چکیده
منابع مشابه
First appearance and development of motile properties in outer hair cells isolated from guinea-pig cochlea.
Cochleae from fetal guinea-pigs (37 to 64 gestation days, gd) were used to correlate the appearance of motile properties of isolated outer hair cells (OHCs) with the development of specific morphological features. Both the 'fast' electrically-driven and the 'slow' calcium-induced motilities appeared first in OHCs from basal turn of 52 gd fetuses. At 56 gd, most of basal and some apical OHCs res...
متن کاملThe effect of pressure on potassium currents in the inner hair cells isolated from guinea-pig cochlea.
An increase in hydrostatic pressure in the endolymphatic system causes hydrops-related inner ear diseases such as Meniere's disease or low tone sensorineural hearing loss. In the present study, we investigated the effects of pressure exerted on potassium currents in acutely isolated inner hair cells of the guinea-pig cochlea using whole-cell voltage-clamp techniques. By applying negative or pos...
متن کاملTwo types of voltage-dependent potassium channels in outer hair cells from the guinea pig cochlea.
Cell-attached and cell-free configurations of the patch-clamp technique were used to investigate the conductive properties and regulation of the major K+channels in the basolateral membrane of outer hair cells freshly isolated from the guinea pig cochlea. There were two major voltage-dependent K+ channels. A Ca2+-activated K+ channel with a high conductance (220 pS, P K/ P Na= 8) was found in a...
متن کاملTwo types of voltage-dependent potassium channels in outer hair cells from the guinea pig cochlea
Van Den Abbeele, Thierry, Jacques Teulon, and Patrice Tran Ba Huy. Two types of voltage-dependent potassium channels in outer hair cells from the guinea pig cochlea. Am. J. Physiol. 277 (Cell Physiol. 46): C913–C925, 1999.— Cell-attached and cell-free configurations of the patch-clamp technique were used to investigate the conductive properties and regulation of the major K1 channels in the bas...
متن کاملTemperature Enhances Activation and Inactivation Kinetics of Potassium Currents in Inner Hair Cells Isolated from Guinea-Pig Cochlea
OBJECTIVES Until recently, most patch-clamp recordings in inner hair cells (IHCs) have been performed at room temperature. The results acquired at room temperature should be corrected if they are to be related to in vivo findings. However, the temperature dependency to ion channels in IHCs is unknown. The aim of this study was to investigate the effect of temperature on the potassium currents i...
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ژورنال
عنوان ژورنال: Practica Oto-Rhino-Laryngologica
سال: 1976
ISSN: 1884-4545,0032-6313
DOI: 10.5631/jibirin.69.1719